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revert 700 destaining solution  (LI-COR)


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    Structured Review

    LI-COR revert 700 destaining solution
    Revert 700 Destaining Solution, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/revert+700+destaining+solution/REVERT+700+WASH+SOLUTION/pmc12907856-91-17-21
    Average 96 stars, based on 52 article reviews
    revert 700 destaining solution - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Imaging:

    Article Title: Probe-dependent Proximity Profiling (ProPPr) Uncovers Similarities and Differences in Phospho-Tau-Associated Proteomes Between Tauopathies.
    Article Snippet: After protein transfer, the membranes were dried for 10 min at 37 °C and stained using the Revert 700 Total Protein Staining Kit (926–11,010, LI-COR) for normalization, according to the manufacturer’s protocol. .. Membranes were scanned using the Odyssey DLx Imaging System (LI-COR) and subsequently de-stained with the Revert 700 destaining solution (926–11,023, LI-COR). .. The membranes were then blocked with Intercept PBS Blocking Buffer (927–70,001, LI-COR) for 1 h at room temperature and incubated overnight at 4 °C with IRDye 800CW-conjugated streptavidin (1:10,000, 926–32,230, LI-COR).

    Article Title: Probe-dependent Proximity Profiling (ProPPr) Uncovers Similarities and Differences in Phospho-Tau-Associated Proteomes Between Tauopathies
    Article Snippet: After protein transfer, the membranes were dried for 10 min at 37 °C and stained using the Revert 700 Total Protein Staining Kit (926–11,010, LI-COR) for normalization, according to the manufacturer’s protocol. .. Membranes were scanned using the Odyssey DLx Imaging System (LI-COR) and subsequently de-stained with the Revert 700 destaining solution (926–11,023, LI-COR). .. The membranes were then blocked with Intercept PBS Blocking Buffer (927–70,001, LI-COR) for 1 h at room temperature and incubated overnight at 4 °C with IRDye 800CW-conjugated streptavidin (1:10,000, 926–32,230, LI-COR).

    Western Blot:

    Article Title: Orphan Nuclear Receptor Family 4A (NR4A) Members NR4A2 and NR4A3 Selectively Modulate Elements of the Monocyte Response to Buffered Hypercapnia
    Article Snippet: Wet transfer was performed onto nitrocellulose membranes and reversibly stained with Revert 700 total protein stain (Li-Cor, Lincoln, NE, USA). .. Membranes were imaged at 700 nm, washed in Revert 700 wash solution (Li-Cor, Lincoln, NE, USA) and destained in Revert 700 destaining solution (Li-Cor, Lincoln, NE, USA) prior to immunoblotting with the ETS-1 antibody (Cell Signalling Technology, Danvers MA, USA #14069), or Lamin (Cell Signalling Technology, Danvers MA, USA, #4777) antibody. .. Fluorescent secondary goat anti-mouse or anti-rabbit antibody incubations were performed (DyLight TM 800, ThermoFisher Scientific (Invitrogen), Waltham, MA, USA) and imaged at 800 nm (1:2000 dilution).

    Article Title: Hypercapnia alters mitochondrial gene expression and acylcarnitine production in monocytes.
    Article Snippet: Wet transfer was performed onto nitrocellulose membranes and reversibly stained with Revert 700 total protein stain (Li-COR, Lincoln, NE, USA). .. Membranes were imaged at 700 nm, washed in Revert 700 wash solution (Li-COR) and destained in Revert 700 destaining solution (Li-Cor) prior to immunoblotting with the MitoProfile Total OXPHOS Human WB Antibody Cocktail (ab110411; Abcam (Cambridge, UK); 1:650–1:1000 dilution), p100/p52 antibody (CST4882; Cell signaling technologies (CST), Danvers, MA, USA) 1:1000 dilution) or RIP antibody (CST3493) 1:1000 dilution. .. Fluorescent secondary goat anti-mouse or anti-rabbit antibody incubations were performed (DyLight 800, Thermo Fisher) and imaged at 800 nm (1:2000 dilution).

    Article Title: Activity-based CO 2 sensing using CarboSenR2 provides new insights into cellular metabolism
    Article Snippet: Wet transfer was performed onto nitrocellulose membranes and reversibly stained with Revert 700 total protein stain (LI-COR, Lincoln, NE, USA). .. Membranes were washed in Revert 700 wash solution and imaged at 700 nm (LI-COR), then destained in Revert 700 destaining solution (Li-Cor) prior to immunoblotting. .. Membranes were blocked in 5% milk in TBST for 1 h followed by overnight incubation in primary antibody (1:1000) at 4 °C (phospho Acetyl-CoA Carboxylase (Ser79) (CST-3661S, Cell Signalling), PGC-1a (A12348, ABclonal), Lamin A/C (CST-4777S, Cell Signalling), mitochondrial cytochrome c oxidase subunit 1 (ab14705, Abcam)).

    Article Title: Activity-based CO 2 sensing using CarboSenR2 provides new insights into cellular metabolism.
    Article Snippet: Wet transfer was performed onto nitrocellulose membranes and reversibly stained with Revert 700 total protein stain (LI-COR, Lincoln, NE, USA). .. Membranes were washed in Revert 700 wash solution and imaged at 700 nm (LICOR), then destained in Revert 700 destaining solution (Li-Cor) prior to immunoblotting. .. Membranes were blocked in 5% milk in TBST for 1 h followed by overnight incubation in primary antibody (1:1000) at 4 ◦C (phospho Acetyl-CoA Carboxylase (Ser79) (CST-3661S, Cell Signalling), PGC-1a (A12348, ABclonal), Lamin A/C (CST-4777S, Cell Signalling), mitochondrial cytochrome c oxidase subunit 1 (ab14705, Abcam)).



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